Υ干扰素对人脐带间充质干细胞生物学特性和免疫调节的作用
Υ干扰素以浓度依赖形式抑制人脐带间充质干细胞(HUC-MSC)的SSEA4表达，增强CD54的表达，促进外周血单个核细胞的增殖。Υ干扰素明显降低HUC-MSC分泌的PGE2。在基因水平上Υ干扰素显著提高IDO-1，降低COX-2水平，IDO-2保持不变。
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Apr;20(2):421-6.
[Effects of interferon-γ on biological characteristics and immunomodulatory property of human umbilical cord-derived mesenchymal stem cells].
[Article in Chinese]
Rong LJ, Chi Y, Yang SG, Chen DD, Chen F, Xu SX, Zhang DL, Ma FX, Lu SH, Han ZC.
Source
Chinese Academy of Medical Sciences, Tianjin, China.
Abstract
The aim of this study was to investigate the effects of interferon (IFN)-γ on biological characteristics and immunomodulatory property of human umbilical cord-derived mesenchymal stem cells (hUC-MSC). hUC-MSC were treated with IFN-γ 10 ng/ml (IFN-γ group) or without IFN-γ (control group). The phenotype of hUC-MSC was detected by flow cytometry. The proliferation status was detected by CCK-8 method, and its differentiation ability was assessed by oil red O and von Kossa staining. The production of PGE-2 was measured by ELISA, and the mRNA expression levels of COX-2, IDO-1 and IDO-2 in hUC-MSC were detected by real-time quantitative PCR. Furthermore, the proliferation of human peripheral blood mononuclear cells (hPBMNC) was evalsuated after co-culture with hUC-MSC, IFN-γ pretreatment or not. The results showed that after IFN-γ stimulation, the expression of SSEA-4 on hUC-MSC decreased significantly [(8.15 ± 2.94) vs (16.42 ± 8.5), P < 0.05], and the expression of CD54 increased [(96.64 ± 3.29) vs (84.12 ± 10.73), P = 0.051]. The immunomodulatory property of hUC-MSC on the proliferation of hPBMNC was enhanced (P < 0.05). All the above mentioned effects were IFN-γ concentration-dependent. When hUC-MSC were stimulated by IFN-γ for 24 h, the production of PGE-2 secreted by hUC-MSC decreased significantly (P < 0.01). The mRNA expression level of COX-2 also decreased though the difference did not reach to statistically significant level. Compared with control group, IDO-1 expression level in IFN-γ group increased significantly (P < 0.01), and the mRNA expression level of IDO-2 remained unchanged. It is concluded that IFN-γ can influence the phenotype of hUC-MSC and enhance the immunomodulatory property of hUC-MSC.
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