为临床应用间充质干细胞（MSC）更安全，检测体外培养扩增的MSC的基因稳定性，冻存稳定性，以及血清在制备MSC过程中的作用。证明在长期体外扩增过程中会发生基因突变，所以建议临床应用MSC 应选择早期代次的细胞为优。

Stem Cells Int. 2012;2012:652034. doi: 10.1155/2012/652034. Epub 2012 May 16.

Safety of mesenchymal stem cells for clinical application.

Wang Y, Han ZB, Song YP, Han ZC.

Source

State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union of Medical College, Tianjin 300020, China.

Abstract

Mesenchymal stem cells (MSCs) hold great promise as therapeutic agents in regenerative medicine and autoimmune diseases, based on their differentiation abilities and immunosuppressive properties. However, the therapeutic applications raise a series of questions about the safety of culture-expanded MSCs for human use. This paper summarized recent findings about safety issues of MSCs, in particular their genetic stability in long-term in vitro expansion, their cryopreservation, banking, and the role of serum in the preparation of MSCs.