人脐带间充质干细胞的条件培养基对体外造血的支持作用
利用人脐带间充质干细胞的培养上清液作为条件培养基，观察其对CD34+细胞的支持作用，发现单独的人脐带间充质干细胞条件培养基就有支持体外造血的能力，但同时促进CD34+细胞向髓系而不是红系分化。
延伸：造血干细胞移植总是受限于干细胞的数量。如果能够在体外扩增得到足够多的免疫匹配的造血干细胞将大大提高移植成功率。越来越多研究证明间充质干细胞对造血功能的支持。那么是选择在体外扩增造血干细胞后移植，还是在造血干细胞移植时共移植间充质干细胞，需要斟酌。本文的结果提示共移植的效果也许更好。
5. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Aug;20(4):975-80.
[Supportive effects of conditioned culture media of human umbilical cord mesenchymal stem cells on hematopoiesis in vitro].
[Article in Chinese]
Li LN, Han ZB, Wang YW, Luo WF, Ji YR, Yang ZX, Feng L, Qi RB, Li YQ, Han ZC.
Source
School of Medicine, Jinan University, Guangzhou, Guangdong Province, China.
Abstract
This study was aimed to explore whether the conditioned culture medium of human umbilical cord-derived mesenchymal stem cells (hUC-MSC) has supportive effects on hematopoiesis in vitro. hUC-MSC were cultured in 75 cm(2) culture flasks at a concentration of 2×10(6) cells per flask. After 48 h, the conditioned culture medium was harvested. CD34(+) cells were isolated with the human cord blood CD34 positive selection kit. The CD34(+) cells were plated in three different culture systems: the culture supernatant from hUC-MSC added into incomplete methylcellulose without recombinant human cytokines as conditioned culture medium; the complete methylcellulose medium with recombinant human cytokines as positive control medium; incomplete methylcellulose adding DMEM/F12 with 10% FBS instead of conditioned culture medium as the negative control medium. After 14 days of culture, colonies containing ≥ 50 cells were scored and types of colonies were classified under inverted microscope. The immunophenotypes of cells which were collected from the colonies were detected by flow cytometry. The results showed that conditioned culture medium of hUC-MSC supported the differentiation of CD34(+) cells into CFU-G (47.67 ± 0.58), CFU-GM (48.67 ± 4.73) and CFU-M (3.00 ± 2.00) in vitro, while the CFU-E, BFU-E or CFU-GEMM were absent. Comparatively, in the positive control medium all kinds of CFU were observed. Interestingly, the percentage of CD45(+)cells of CFU in conditioned culture medium (97.43 ± 2.15)% was more than CD45(+)cells in positive control medium (39.69 ± 0.96)% (P < 0.05). It is concluded that the conditioned culture medium of hUC-MSC has been confirmed to have ability to support hematopoiesis separately in vitro. Besides, it enhances the differentiation of CD34(+) cells into myeloid cells except cells of erythroid lineage.
