诱导胚胎胰腺干细胞分化为成熟的内分泌细胞的新方法
本文建立了一种新方法，能够使胰腺胚胎细胞有效分化为成熟的内分泌细胞，并且其胰腺细胞标记谱未发生改变。
延伸：胰岛素治疗并不能防止1型糖尿病发展成包括严重的血管疾病、神经疾病、视网膜病和肾衰竭等综合症。对于胚胎胰腺干细胞的研究有助于对该疾病的了解，进而提供可能的治疗方法。
4. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2012 Aug;34(4):343-7. doi: 10.3881/j.issn.1000-503X.2012.04.006.
Establishment of a new method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells.
Chen F, Ma FX, Chi Y, Zhao QJ, Yang SG, Lu SH, Han ZC.
Source
Institute of Hematology and Hospitalof Blood Diseases, CAMS and PUMC, Tianjin, China.
Abstract
OBJECTIVE:
To establish a new culture method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells.
METHODS:
Mouse embryos at day 12.5 were used and embryonic pancreata were isolated. The isolated embryonic pancreata were cultured on the filter for 7 days, which floated in the dish containing medium. During culture, the expression of pancreas duodenum homeobox-1 (PDX-1), a pancreatic stem cell marker, was examined at day 1. The expression of neurogenin 3 (Ngn3), a pancreatic progenitor cell marker, was examined at day 3. The expressions of endocrine and exocrine markers, insulin, glucagon, and carboxypeptidase (CPA) were examined at day 7 by immunohistochemistry. The kinetics of pancreatic marker expression during culture was assayed by real-time PCR.
RESULTS:
Many pancreatic stem cells still existed in embryonic pancreata cultured for 1 day; meanwhile, these pancreatic stem cells proliferated in high rate. A large amount of pancreatic progenitor cells were found in embryonic pancreata cultured for 3 days.Pancreatic stem/progenitor cells differentiated into mature endocrine and exocrine cells in embryonic pancreata after having been cultured for 7 days. Furthermore, the expression pattern of pancreatic marker is consistent with that in vivo.
CONCLUSION:
We successfully established a new culture method, with which embryonic pancreatic cells can efficiently differentiate into mature endocrine cell.
