人脐带来源的间充质干细胞对前房相关行免疫异常的作用
国际免疫药学 2013 1；15（1）：114-20
人脐带来源的间充质干细胞对前房相关行免疫异常的作用。
在前房相关免疫异常的小鼠模型中，静脉注射间充质干细胞加强IL-10和TGF-β产生，进一步抑制脾脏单核细胞IFN-γ分泌，增强CD4(+)CD25(+)Foxp3(+) 和 CD8(+)Foxp3(+) Tregs细胞的扩增，可能有助于前房相关性免疫异常病程迁延。
延伸：虽然自身免疫性疾病应用间充质干细胞进行治疗取得一定进展，但是对于免疫系统疾病引用间充质干细胞，应该谨慎选择适用症。
Int Immunopharmacol. 2013 Jan;15(1):114-20. doi: 10.1016/j.intimp.2012.11.007. Epub 2012 Nov 21.
Effects of human umbilical cord-derived mesenchymal stem cells on anterior chamber-associated immune deviation.
Zhang Y, Zhang M, Zhao S, Li X, Jia Z, Zhang L, Han ZC, Zhang X.
Source
Tianjin Medical University Eye Hospital and Eye Institute, 251 Fu Kang Road, Tianjin, China.
Abstract
Introduction of antigen into anterior chamber (AC) induces a deviant immune response termed anterior chamber-associated immune deviation (ACAID) that protects the eye from inflammatory destruction consequent to a systemic immune response. Mesenchymal stem cells (MSCs) can modulate a variety of immune responses. However, the effects of systemic administration of MSCs on ACAID have not been explored. In this study, C57BL/6 mice were inoculated with ovalbumin in the AC to induce ACAID, control group received AC injection of solvent alone. Immediately after the AC injection, the mice were injected through the tail vein with human Umbilical Cord-derived MSCs (hUC-MSC) or phosphate buffer saline. All animals were subcutaneously immunized with ovalbumin one week later. Delayed-type hypersensitivity assay was performed another week following immunization. The splenic monocytes were then isolated, cultured and stimulated with ovalbumin. Levels of IL-10, TGF-β, and IFN-γ in culture media were measured by ELISA. The frequencies of CD4(+)CD25(+)Foxp3(+) and CD8(+)Foxp3(+) regulatory T cells (Tregs) were determined by flow cytometry. The results showed that the AC inoculation of ovalbumin induced significantly less ear swelling than controls, confirming the establishment of ACAID. MSCs potentiated IL-10 and TGF-β production, further suppressed IFN-γ secretion from splenic monocytes in ACAID mice, and enhanced expansion of CD4(+)CD25(+)Foxp3(+) and CD8(+)Foxp3(+) Tregs isolated from the spleen of ACAID mice. Therefore, our study, for the first time, provides clear evidence that systemic administration of MSCs augments cytokine production and Treg expansion from ACAID spleens, which may contribute to promotion and maintenance of ACAID.
